关键词: γ辐射, 1H NMR谱, 代谢产物, 时间相关性, 尿

Abstract: OBJECTIVE: To investigate effects of urinary metabolites from single exposure to γ radiation and to screen urinary metabolites related close to γ radiation damage in rats. METHODS：15 male adult SD rats were exposed to 60Coγ-rays whole body once at 6.0 Gy，0.7 Gy/min. Urine was collected from rats one day before irradiation and 1 d，2 d，3 d，4 d，8 d，18 d，30 d and 45 d after irradiation. Urine 1H NMR spectra were acquired，principal component analysis (PCA) and partial least-squares-discriminant analysis (PLS-DA) methods are utilized to analyze 1H NMR spectra data. Then we looked for the metabolites that contributed more in the difference between urine 1H NMR spectra before exposure and every time after exposure( abbreviateed as common difference metabolites) and analyzed relation between the change of their relative contents and post-irradiation time. RESULTS：60Coγ irradiation could cause visible change of urine 1H NMR spectra. Common difference metabolites were taurine，trimethylamine-N-oxide，proline，creatinine，citrate，succinate，acetate and lactate that coutributed more in change of 1H NMR spectra of urine collected at different times after exposure. For 1-45 d after exposure，the relative contents of proline and taurine were markedly higher than before irradiation，and citrate and succinate were lower. However，the relative contents of trimethylamine-N-oxide，acetate and lactate first increased 1 d after irradiation and then decreased for 2-45 d after irradiation. CONCLUSION：The disorder of metabolism in rats induced by high-dose γ radiation did not completely recover for 45 days after irradiation，that showed persistent changes of the relative contents of urine taurine， proline，creatinine，citrate，succinate，acetate and lactate in exposed rats. Taurine and proline could become potential combined metabolic markers of acute high-dose radiation injury.

Key words: γ radiation, 1H NMR spectra, metabolites, correlation with time, urine